Designed for human reference gene (β actin) nucleic acid detection, this kit enables efficient and accurate detection of human β actin, making it widely applicable for both qualitative and quantitative β actin analysis. The product does not include live samples as positive controls; instead, it provides synthetically produced specific DNA fragments as positive standards. This kit is intended strictly for scientific research by professionals and is not for use in clinical diagnosis or treatment.
PRODUCTS
The Human Reference Gene (β actin) nucleic acid detection kit (fluorescent PCR method) achieves quantitative detection of β actin nucleic acid through a fluorescent probe combined with a standard curve. This PCR detection system includes a pair of specific primers and one specific fluorescent probe. It utilizes It utilizes advanced hot-start DNA polymerase, which enhances PCR amplification efficiency. This makes it suitable for high-sensitivity amplification of low-concentration pathogen nucleic acids.
This kit employs a specially formulated qPCR buffer and a UNG/dUTP anti-contamination system, ensuring a well-defined standard curve across a broad quantification range for accurate quantification and effective prevention of false positives caused by residual PCR products or aerosol contamination. The kit is compatible with most brands of fluorescent quantitative PCR instruments, including those from Applied Biosystems, Eppendorf, Bio-Rad, and Roche.
With high specificity, the kit can accurately identify the target sequence, minimizing non-specific amplification and cross-reactivity. Additionally, it offers excellent stability, retaining activity and performance over extended storage conditions to ensure reliable and consistent experimental results.
Introduction
Details\Advantages
(1) High Sensitivity and Specificity:
Utilizing TaqMan probe-specific binding, this kit accurately differentiates the target gene from non-target sequences. It offers extremely high sensitivity and accuracy, ensuring reliable quantification results.
(2) Fast and Efficient:
The kit employs advanced TaqMan technology, enabling precise quantification in a short time, significantly reducing the experimental cycle and enhancing experimental efficiency.
(3) High Signal-to-Background Ratio:
The TaqMan probe, with its fluorescent label and internal quencher, provides a high signal contrast, effectively reducing background noise for precise, low-background detection.
(4) High Reproducibility:
This kit consistently delivers stable performance and results in both high-throughput and single-experiment settings, ensuring high reproducibility and reliability in experiments.
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