Saprolegnia ferax(SF) Nucleic Acid Detection Kit (Fluorescent PCR Method) enables quantitative detection of Saprolegnia ferax(SF) nucleic acid using fluorescent probes combined with a standard curve. This PCR detection system includes a pair of specific primers and one specific fluorescent probe. It utilizes It utilizes advanced hot-start DNA polymerase, which enhances PCR amplification efficiency. This makes it suitable for high-sensitivity amplification of low-concentration pathogen nucleic acids.

This kit employs a specially formulated qPCR buffer and a UNG/dUTP anti-contamination system, ensuring a well-defined standard curve across a broad quantification range for accurate quantification and effective prevention of false positives caused by residual PCR products or aerosol contamination. The kit is compatible with most brands of fluorescent quantitative PCR instruments, including those from Applied Biosystems, Eppendorf, Bio-Rad, and Roche.

With high specificity, the kit can accurately identify the target sequence, minimizing non-specific amplification and cross-reactivity. Additionally, it offers excellent stability, retaining activity and performance over extended storage conditions to ensure reliable and consistent experimental results.